Chemoenzymatic synthesis of the bacterial polysaccharide repeating unit undecaprenyl pyrophosphate and its analogs

Lei Li, Robert L Woodward, Weiqing Han, Jingyao Qu, Jing Song, Cheng Ma, Peng G Wang

Nature Protocols · 2016 · 10.1038/nprot.2016.067

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★ 5.0 1 rating
5 : Major extension (new functional group) ×1
4 : Minor extension (no new functional group) –
3 : Reproduced as published –
2 : Reproduced with deviation –
1 : Did not work –
– : Extension failed / Inconclusive –

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Industry / CRO chemist
09 Aug 2026 · edited
★ 5 : Major extension (new functional group)

I have tested most of the reactions described in this paper using closely related, although not identical, substrates, and they generally work well. In some cases, however, it is advisable to reduce the amount of certain reagents. For example, reducing the concentration of NH₄HCO₃ to 0.2 M during the RP purification performed after pyrophosphate formation, or lowering the tetrazole loading during phosphate formation, can be beneficial.